Human V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) ELISA Kit
The Human V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RelB) ELISA Kit offered by AssayGenie is a cutting-edge tool for the precise quantification of RelB levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research studies.RelB is a key protein involved in immune response regulation and inflammatory processes. Its dysregulation has been linked to various diseases, including cancer, autoimmune disorders, and chronic inflammation.
By accurately measuring RelB levels, researchers can gain valuable insights into the pathogenesis of these conditions and identify potential therapeutic targets.Trust AssayGenie's Human V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RelB) ELISA Kit for reliable and comprehensive analysis of RelB levels, paving the way for innovative discoveries and advancements in the field of molecular biology and immunology.
Product Name:
Human V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) ELISA Kit
Product Code:
HUDL02523
Size:
96 Assays
Target:
RELB
Synonyms:
REL-B, I-REL, Transcription Factor RelB, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
