The Human Vaccinia-Related Kinase 1 (VRK1) ELISA Kit is a powerful tool for accurately measuring VRK1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is designed for high sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.VRK1 is a key enzyme that regulates cell cycle progression and DNA damage response, making it a vital player in various cellular processes. Dysregulation of VRK1 has been linked to conditions like cancer, autoimmune diseases, and neurological disorders, highlighting its importance as a potential biomarker for disease progression and therapy development.
By using the Human VRK1 ELISA Kit, researchers can delve deeper into the role of VRK1 in human health and disease, paving the way for innovative diagnostic and therapeutic strategies. Trust in the accuracy and reliability of this kit for all your VRK1 research needs.
Product Name:
Human Vaccinia Related Kinase 1 (VRK1) ELISA Kit
Product Code:
HUDL03105
Size:
96 Assays
Target:
VRK1
Synonyms:
Serine/threonine-protein kinase VRK1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
