Human VAP-1 (Vascular Adhesion Protein 1) ELISA Kit
The Human VAP-1 (Vascular Adhesion Protein-1) ELISA Kit is specifically designed for the precise measurement of VAP-1 levels in human serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research purposes.VAP-1 is a key protein involved in mediating leukocyte adhesion and migration, playing a critical role in inflammatory responses and vascular pathology. Its dysregulation has been linked to various immune disorders, autoimmune diseases, and inflammatory conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
Overall, the Human VAP-1 ELISA Kit provides researchers with a powerful tool for investigating the role of VAP-1 in health and disease, ultimately contributing to advancements in understanding and treating a range of inflammatory and vascular-related disorders.
Product Name:
Human VAP-1 (Vascular Adhesion Protein 1) ELISA Kit
SKU:
HUES03143
Target:
Human VAP-1 (Vascular Adhesion Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VAP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VAP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VAP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VAP-1. You can calculate the concentration of Human VAP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
83-97
92-103
Average (%)
100
90
98
1:4
Range (%)
93-104
80-94
84-95
Average (%)
98
86
90
1:8
Range (%)
88-98
79-89
83-94
Average (%)
93
85
88
1:16
Range (%)
86-98
81-93
81-93
Average (%)
92
87
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-110
100
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
83-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.88
240.69
685.3
104.85
253.32
743.12
Standard deviation
5.62
11.02
25.77
5.33
14.69
39.39
C V (%)
5.46
4.58
3.76
5.08
5.8
5.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VAP-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VAP-1 in samples. No significant cross-reactivity or interference between Human VAP-1 and analogues was observed.
