Human VE-Cadherin (Vascular Endothelial Cadherin) CLIA Kit
The Human VE-Cadherin (Vascular Endothelial Cadherin) CLIA Kit is a powerful tool designed for the precise measurement of VE-cadherin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.VE-cadherin is a critical protein involved in maintaining endothelial cell integrity and regulating vascular permeability.
Dysregulation of VE-cadherin has been implicated in various diseases such as cardiovascular disorders, inflammation, and cancer. Therefore, this kit serves as a valuable resource for investigating the role of VE-cadherin in these conditions and exploring potential therapeutic strategies.
Product Name:
Human VE-Cadherin (Vascular Endothelial Cadherin) CLIA Kit
SKU:
HUES01138
Target:
Human VE-Cadherin (Vascular Endothelial Cadherin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VE-Cadherin. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VE-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VE-Cadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VE-Cadherin. You can calculate the concentration of Human VE-Cadherin in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
86-100
88-101
Average (%)
100
93
93
1:4
Range (%)
102-114
93-108
102-116
Average (%)
108
99
109
1:8
Range (%)
96-110
95-110
94-111
Average (%)
101
103
101
1:16
Range (%)
95-107
93-109
89-99
Average (%)
101
100
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
99
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
93-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.14
196.64
869.59
89.94
205.8
861.05
Standard deviation
11.56
18.43
84.35
11.57
17.43
77.06
C V (%)
11.78
9.37
9.7
12.86
8.47
8.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VE-Cadherin concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VE-Cadherin in samples. No significant cross-reactivity or interference between Human VE-Cadherin and analogues was observed.
