Human VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit
The Human VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit is meticulously developed to enable the precise quantitative detection of VE-Cadherin in various human biological samples. VE-Cadherin, a critical protein involved in endothelial cell adhesion and vascular integrity, plays a significant role in regulating vascular permeability and endothelial barrier function.
Accurate measurement of VE-Cadherin levels is essential for understanding vascular physiology, endothelial function, and vascular-related pathologies. Our VE-Cadherin ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results vital for in-depth research activities. Manufactained under stringent quality control standards, this kit guarantees robust performance, delivering reliable data with ease of use. Trust Assay Genie's VE-Cadherin ELISA Kit for dependable quantification of this essential vascular biomarker in your investigative studies.
Product Name:
Human VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit
SKU:
AEES00137
Target:
Human VE-Cadherin (Vascular Endothelial Cadherin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VE-Cadherin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VE-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VE-Cadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VE-Cadherin. You can calculate the concentration of Human VE-Cadherin in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-96
93-106
91-106
Average (%)
91
100
98
1:4
Range (%)
93-104
94-105
92-106
Average (%)
98
99
100
1:8
Range (%)
84-96
96-102
83-98
Average (%)
91
99
90
1:16
Range (%)
94-101
95-106
96-106
Average (%)
97
98
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-103
100
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
91-101
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.09
238.9
906.46
99.6
241.36
993.71
Standard deviation
4.94
10.75
42.42
5.45
11.54
46.9
C V (%)
5.2
4.5
4.68
5.47
4.78
4.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VE-Cadherin concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VE-Cadherin in samples. No significant cross-reactivity or interference between Human VE-Cadherin and analogues was observed.
