Human VEGF-D (Vascular Endothelial Growth Factor D) CLIA Kit
The Human VEGF-D (Vascular Endothelial Growth Factor-D) CLIA Kit is a powerful tool for researchers looking to analyze VEGF-D levels in human samples with high accuracy and precision. This kit is specifically designed for serum, plasma, and cell culture supernatants, providing reliable results for a variety of research applications.VEGF-D is a key player in promoting the growth of blood vessels and is implicated in various diseases, including cancer and cardiovascular disorders.
By accurately measuring VEGF-D levels, researchers can gain valuable insights into disease progression and potential therapeutic targets.With its high sensitivity and specificity, the Human VEGF-D CLIA Kit offers researchers a dependable tool for studying VEGF-D and its role in angiogenesis. Trust this kit to deliver consistent and reproducible results for your research needs.
Product Name:
Human VEGF-D (Vascular Endothelial Growth Factor D) CLIA Kit
SKU:
HUES00912
Target:
Human VEGF-D (Vascular Endothelial Growth Factor D)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-D. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-D, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VEGF-D. You can calculate the concentration of Human VEGF-D in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-113
101-119
87-98
Average (%)
103
109
92
1:4
Range (%)
89-102
96-111
92-107
Average (%)
94
103
98
1:8
Range (%)
90-107
85-96
101-117
Average (%)
98
91
107
1:16
Range (%)
89-104
103-120
98-112
Average (%)
95
110
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-114
107
EDTA plasma (n=5)
90-101
96
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.7
143.18
386.0
45.77
141.57
380.45
Standard deviation
3.91
13.2
27.25
4.51
16.21
35.88
C V (%)
8.2
9.22
7.06
9.85
11.45
9.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VEGF-D concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VEGF-D in samples. No significant cross-reactivity or interference between Human VEGF-D and analogues was observed.
