Human VEGF-D (Vascular Endothelial Growth Factor D) ELISA Kit
The Human VEGF-D (Vascular Endothelial Growth Factor-D) ELISA Kit is specifically designed for the precise detection of VEGF-D levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for various research applications.VEGF-D is a key protein involved in promoting the growth of blood vessels and influencing cell proliferation. Its role in conditions such as cancer, cardiovascular diseases, and neurodegenerative disorders makes it a valuable biomarker for studying these diseases and exploring potential treatment strategies.
With the Human VEGF-D ELISA Kit, researchers can gain valuable insights into the role of VEGF-D in different disease states and potentially develop new therapeutic approaches. Get reliable and reproducible results with this advanced ELISA kit from Assay Genie.
Product Name:
Human VEGF-D (Vascular Endothelial Growth Factor D) ELISA Kit
SKU:
HUES02610
Target:
Human VEGF-D (Vascular Endothelial Growth Factor D)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-D. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGF-D. You can calculate the concentration of Human VEGF-D in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
96-111
86-99
Average (%)
94
103
91
1:4
Range (%)
101-117
82-94
92-104
Average (%)
107
88
98
1:8
Range (%)
99-114
88-99
95-108
Average (%)
104
93
100
1:16
Range (%)
98-110
83-96
97-114
Average (%)
104
88
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
93-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
200
489.3
1454.2
214.7
534.6
1535.5
Standard deviation
11.4
19.6
72.7
14.4
26.2
66.0
C V (%)
5.7
4.01
5.0
6.71
4.9
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VEGF-D concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VEGF-D in samples. No significant cross-reactivity or interference between Human VEGF-D and analogues was observed.
