The Human VIL1 (Villin-1) CLIA Kit is specifically designed for the precise measurement of Villin-1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it an essential tool for a variety of research applications.Villin-1 is a key protein involved in the structure and function of intestinal epithelial cells, playing a crucial role in maintaining the integrity of the intestinal barrier.
Dysregulation of Villin-1 has been linked to various gastrointestinal disorders, making it an important biomarker for studying these conditions and developing potential treatments.With the Human VIL1 (Villin-1) CLIA Kit, researchers can confidently measure Villin-1 levels in human samples, allowing for a better understanding of intestinal health and disease pathology. This kit is a valuable resource for researchers in the fields of gastroenterology, cell biology, and molecular biology.
Product Name:
Human VIL-1 (Villin 1) CLIA Kit
SKU:
HUES01278
Target:
Human VIL-1 (Villin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VIL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VIL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VIL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VIL1. You can calculate the concentration of Human VIL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
96-111
89-104
Average (%)
90
103
95
1:4
Range (%)
90-106
87-100
86-98
Average (%)
98
93
93
1:8
Range (%)
93-109
89-101
90-102
Average (%)
101
95
97
1:16
Range (%)
101-117
91-105
86-99
Average (%)
109
98
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
95
EDTA plasma (n=5)
99-110
105
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.6
226.14
907.12
95.02
248.31
901.04
Standard deviation
10.53
23.04
91.17
11.16
28.98
90.28
C V (%)
10.68
10.19
10.05
11.74
11.67
10.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VIL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VIL1 in samples. No significant cross-reactivity or interference between Human VIL1 and analogues was observed.
