Human VLDLR (Very Low Density Lipoprotein Receptor) ELISA Kit
The Human VLDLR (Very Low Density Lipoprotein Receptor) ELISA Kit is a highly accurate tool for measuring VLDLR levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.VLDLR is a key receptor involved in lipid metabolism and plays a crucial role in regulating cholesterol and triglyceride levels.
Dysregulation of VLDLR has been linked to various metabolic disorders, cardiovascular diseases, and neurodegenerative conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Human VLDLR ELISA Kit from Assay Genie provides researchers with a reliable and efficient method for quantifying VLDLR levels, facilitating in-depth investigations into its role in health and disease.
Product Name:
Human VLDLR (Very Low Density Lipoprotein Receptor) ELISA Kit
SKU:
HUES02500
Target:
Human VLDLR (Very Low Density Lipoprotein Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VLDLR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VLDLR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VLDLR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VLDLR. You can calculate the concentration of Human VLDLR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
99-113
98-114
Average (%)
104
105
104
1:4
Range (%)
94-106
86-98
88-102
Average (%)
99
91
94
1:8
Range (%)
92-107
82-95
83-97
Average (%)
99
89
88
1:16
Range (%)
93-107
82-96
83-93
Average (%)
98
88
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
100
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.88
30.87
89.76
11.78
28.38
90.9
Standard deviation
0.7
1.49
3.46
0.64
1.26
4.67
C V (%)
6.43
4.83
3.85
5.43
4.44
5.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VLDLR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VLDLR in samples. No significant cross-reactivity or interference between Human VLDLR and analogues was observed.
