Human Von Willebrand Factor A Domain Containing Protein 3A (vWA3A) ELISA Kit
The Human von Willebrand Factor A Domain Containing Protein 3A (VWA3A) ELISA Kit is specifically designed for the precise quantification of VWA3A levels in human serum, plasma, and cell culture supernatants. This kit features exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.VWA3A is a protein containing the von Willebrand factor A domain, which is involved in cell adhesion and signal transduction.
Studies have shown that abnormalities in VWA3A expression are associated with certain diseases, making it a valuable biomarker for investigating these conditions and developing potential treatments.This ELISA kit provides researchers with a reliable tool for studying the role of VWA3A in various physiological and pathological processes, ultimately advancing our understanding of the molecular mechanisms governing health and disease.
Product Name:
Human Von Willebrand Factor A Domain Containing Protein 3A (vWA3A) ELISA Kit
Product Code:
HUDL03112
Size:
96 Assays
Target:
vWA3A
Detection Method:
Sandwich
Range:
0.468-30ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
