The Human Versican PG-M CLIA Kit is specifically designed for the precise measurement of Versican PG-M levels in human cell lysates, serum, and plasma samples. This advanced kit offers high sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Versican PG-M is a key protein involved in extracellular matrix organization and cell adhesion, playing a critical role in tissue development and homeostasis. Dysregulation of Versican PG-M expression has been implicated in various pathological conditions, such as cancer, cardiovascular diseases, and inflammatory disorders, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.
By utilizing the Human Versican PG-M CLIA Kit, researchers can uncover new insights into the role of Versican PG-M in disease progression and potentially identify novel targets for diagnosis and treatment. Order your kit today to advance your research efforts in the field of molecular biology and biomedicine.
Product Name:
Human VS (Versican/PG-M) CLIA Kit
SKU:
HUES00703
Target:
Human VS (Versican/PG-M)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VS. You can calculate the concentration of Human VS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
97-114
101-117
Average (%)
109
105
109
1:4
Range (%)
95-110
98-113
85-95
Average (%)
103
104
90
1:8
Range (%)
100-114
88-102
101-114
Average (%)
108
93
108
1:16
Range (%)
90-101
89-101
99-115
Average (%)
95
96
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
100-116
106
Cell culture media (n=5)
100-114
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
194.64
410.5
1592.99
189.06
381.41
1510.62
Standard deviation
17.36
32.35
106.57
21.02
35.51
99.55
C V (%)
8.92
7.88
6.69
11.12
9.31
6.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VS in samples. No significant cross-reactivity or interference between Human VS and analogues was observed.
