The Human Versican (PG-M) ELISA Kit is specifically designed for the precise measurement of Versican (PG-M) levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Versican (PG-M) is a key protein involved in extracellular matrix organization and cell behavior regulation. It plays a critical role in various biological processes such as cell adhesion, migration, proliferation, and tissue development.
Dysregulation of Versican (PG-M) expression has been associated with various diseases, including cancer, cardiovascular diseases, and inflammatory disorders, making it a valuable biomarker for disease research and therapeutic development.Overall, the Human Versican (PG-M) ELISA Kit provides researchers with a reliable tool to study the role of Versican (PG-M) in physiological and pathological processes, offering insights into potential therapeutic targets for a range of diseases.
Product Name:
Human VS (Versican/PG-M) ELISA Kit
SKU:
HUES02300
Target:
Human VS (Versican/PG-M)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VCAN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VCAN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VCAN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VCAN. You can calculate the concentration of Human VCAN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
87-104
90-103
Average (%)
95
95
95
1:4
Range (%)
91-106
86-100
84-97
Average (%)
97
92
89
1:8
Range (%)
91-103
87-100
87-99
Average (%)
97
92
92
1:16
Range (%)
90-105
82-93
88-100
Average (%)
97
88
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
92
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
2.97
8.7
0.87
3.16
9.15
Standard deviation
0.05
0.13
0.39
0.06
0.17
0.38
C V (%)
5.43
4.38
4.48
6.9
5.38
4.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VCAN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VCAN in samples. No significant cross-reactivity or interference between Human VCAN and analogues was observed.
