The Human VTN (Vitronectin) ELISA Kit is a specialized assay designed to quantitatively detect Vitronectin levels in various biological samples. Vitronectin is a multifunctional glycoprotein involved in cell adhesion, spreading, and migration, as well as in regulating physiological and pathological processes such as hemostasis and tissue remodeling. Accurate measurement of VTN levels is essential for understanding its roles in various cellular processes and diseases.
Our VTN ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. This kit is manufactured under stringent quality control standards to deliver robust performance while being user-friendly, making it suitable for both research and clinical applications. Trust Assay Genie's VTN ELISA Kit for precise and dependable quantification of this vital biomarker in your studies.
Product Name:
Human VTN (Vitronectin) ELISA Kit
SKU:
AEES00100
Target:
Human VTN (Vitronectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VTN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VTN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VTN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VTN. You can calculate the concentration of Human VTN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-108
88-101
90-102
Average (%)
105
96
96
1:4
Range (%)
101-107
101-110
87-101
Average (%)
104
105
94
1:8
Range (%)
97-111
90-97
90-100
Average (%)
103
93
95
1:16
Range (%)
93-102
89-102
87-100
Average (%)
97
94
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-110
105
EDTA plasma (n=5)
91-99
96
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.36
123.68
471.65
40.22
121.02
478.15
Standard deviation
2.19
5.6
23.87
2.06
5.49
25.01
C V (%)
5.05
4.53
5.06
5.12
4.54
5.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VTN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VTN in samples. No significant cross-reactivity or interference between Human VTN and analogues was observed.
