SynonymsWFDC2,HE4,WAP5,EDDM4,Epididymal Protein 4,Epididymal Secretory Protein E4,Putative Protease Inhibitor WAP5,Major epididymis-specific protein E4
ReactivityHuman
Sample TypeSerum, Plasma, Saliva, Tissue Homogenates And Other Biological Fluids
SKU:
HUDL03116
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q14508
Sensitivity:
0.068ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
WFDC2,HE4,WAP5,EDDM4,Epididymal Protein 4,Epididymal Secretory Protein E4,Putative Protease Inhibitor WAP5,Major epididymis-specific protein E4
Reactivity:
Human
Sample Type:
Serum, Plasma, Saliva, Tissue Homogenates And Other Biological Fluids
Human WAP Four Disulfide Core Domain Protein 2 (WFDC2) ELISA Kit
The Human WFDC2 (WAP Four-Disulfide Core Domain Protein 2) ELISA Kit is a powerful tool for the quantitative measurement of WFDC2 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.WFDC2 is a critical protein involved in various physiological processes, including immune regulation, inflammation, and cancer progression.
Understanding the levels of WFDC2 in biological samples can provide valuable insights into disease mechanisms and potential therapeutic targets. The Human WFDC2 ELISA Kit is an indispensable tool for researchers studying conditions related to WFDC2, offering reliable and precise measurements for a wide range of applications.
Product Name:
Human WAP Four Disulfide Core Domain Protein 2 (WFDC2) ELISA Kit
Product Code:
HUDL03116
Size:
96 Assays
Synonyms:
WFDC2, HE4, WAP5, EDDM4, Epididymal Protein 4, Epididymal Secretory Protein E4, Putative Protease Inhibitor WAP5, Major epididymis-specific protein E4
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
