Human Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ELISA Kit
The Human Wingless-Type MMTV Integration Site Family Member 10B (WNT10B) ELISA Kit is a specialized assay designed for the accurate and sensitive detection of WNT10B levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity and reproducibility, ensuring reliable results for a variety of research applications.WNT10B is a key member of the Wnt signaling pathway, playing a critical role in various cellular processes including cell proliferation, differentiation, and tissue development.
Dysregulation of WNT10B has been linked to a range of diseases, including cancer, metabolic disorders, and skeletal abnormalities, making it a valuable biomarker for understanding disease mechanisms and potential therapeutic interventions.With its advanced technology and ease of use, the Human WNT10B ELISA Kit is an essential tool for researchers studying WNT signaling pathways, tissue development, and disease pathogenesis. Don't miss out on the opportunity to enhance your research with this innovative and reliable assay.
Product Name:
Human Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ELISA Kit
Product Code:
HUDL03123
Size:
96 Assays
Target:
WNT10B
Synonyms:
WNT-12, SHFM6
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-102
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
