Human Wingless Type MMTV Integration Site Family, Member 2 (WNT2) ELISA Kit
The Human WNT2 (Wingless-Type MMTV Integration Site Family Member 2) ELISA Kit offered by Assay Genie is a powerful tool for researchers looking to accurately measure WNT2 levels in human samples. This kit is compatible with serum, plasma, and cell culture supernatants, providing versatility for a variety of experimental setups. WNT2 is a key player in the Wnt signaling pathway, which is involved in cell proliferation, differentiation, and migration. Dysregulation of WNT2 is linked to various diseases, including cancer and developmental disorders, making it a valuable target for research studies.
With its high sensitivity and specificity, the Human WNT2 ELISA Kit from Assay Genie delivers precise and reliable results, enabling researchers to gain valuable insights into the role of WNT2 in health and disease. Don't miss out on this essential tool for your research endeavors. Visit the Assay Genie website to learn more about our cutting-edge ELISA kits.
Product Name:
Human Wingless Type MMTV Integration Site Family, Member 2 (WNT2) ELISA Kit
Product Code:
HUDL03127
Size:
96 Assays
Target:
WNT2
Synonyms:
IRP, INT1L1, Secreted Growth Factor, Int-1-related protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
