Human Wingless Type MMTV Integration Site Family, Member 7B (WNT7B) ELISA Kit
The Human Wingless-Type MMTV Integration Site Family Member 7B (WNT7B) ELISA Kit is designed to accurately measure WNT7B levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results for a variety of research applications.WNT7B is a member of the WNT signaling pathway that plays a crucial role in regulating cell proliferation, differentiation, and migration.
Dysregulation of WNT signaling has been linked to various diseases such as cancer, developmental disorders, and inflammatory diseases, making WNT7B a valuable biomarker for studying these conditions and potential therapeutic interventions.Get comprehensive insights into WNT7B levels and its impact on biological processes with the Human WNT7B ELISA Kit, an essential tool for cutting-edge research in molecular biology and disease mechanisms.
Product Name:
Human Wingless Type MMTV Integration Site Family, Member 7B (WNT7B) ELISA Kit
Product Code:
HUDL03134
Size:
96 Assays
Target:
WNT7B
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-100
95
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
