HumanWISP1 (WNT1 Inducible Signaling Pathway Protein 1) ELISA Kit
The Human WISP1 (Wnt1 inducible signaling pathway protein 1) ELISA Kit is a valuable tool for detecting WISP1 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an ideal choice for a wide range of research applications.WISP1 is a key player in the Wnt signaling pathway, regulating cell growth, migration, and differentiation.
Its dysregulation has been linked to various diseases, including cancer, fibrosis, and inflammatory disorders. By measuring WISP1 levels, researchers can gain insights into disease mechanisms and identify potential therapeutic targets.Overall, the Human WISP1 ELISA Kit is a reliable solution for studying the role of WISP1 in health and disease, offering researchers a powerful tool to advance their research efforts.
Product Name:
HumanWISP1 (WNT1 Inducible Signaling Pathway Protein 1) ELISA Kit
SKU:
HUES03519
Target:
HumanWISP1 (WNT1 Inducible Signaling Pathway Protein 1) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human WISP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human WISP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human WISP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human WISP1. You can calculate the concentration of Human WISP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
94-109
97-109
Average (%)
97
100
103
1:4
Range (%)
87-99
85-97
85-100
Average (%)
93
90
91
1:8
Range (%)
87-98
87-98
83-93
Average (%)
92
92
88
1:16
Range (%)
92-104
81-96
86-98
Average (%)
98
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
93-108
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
227.76
766.45
2132.87
205.65
798.91
2247.01
Standard deviation
11.48
37.25
83.18
10.82
33.23
68.98
C V (%)
5.04
4.86
3.9
5.26
4.16
3.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human WISP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human WISP1 in samples. No significant cross-reactivity or interference between Human WISP1 and analogues was observed.
