The HUS1B Antibody (CAB6817) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is specifically reactive with human samples and is validated for use in Western blot applications. By binding to the HUS1B protein, this antibody enables the detection and analysis of HUS1B in various cell types, making it an essential tool for studies in DNA repair mechanisms and cancer research.HUS1B is a crucial player in maintaining genome stability by sensing DNA damage and initiating repair processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HUS1B Antibody
SKU:
CAB6817
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HUS1B
Positive Sample:
LO2, Jurkat, Mouse spleen, Mouse thymus, Rat testis, Rat thymus
Cellular Localization:
Nucleolus.
Calculated MW:
31kDa
Observed MW:
31kDa
The protein encoded by this gene is most closely related to HUS1, a component of a cell cycle checkpoint protein complex involved in cell cycle arrest in response to DNA damage. This protein can interact with the check point protein RAD1 but not with RAD9. Overexpression of this protein has been shown to induce cell death, which suggests a related but distinct role of this protein, as compared to the HUS1.
Purification Method
Affinity purification
Gene ID
135458
RRID
AB_2767394
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HUS1B Rabbit pAb (CAB6817) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
