Description
| Product Name: | Hydroxyproline (HYP) Colorimetric Assay Kit |
| Product Code: | MAES0211 |
| Sample Type: | Serum, Animal Tissue, Urine |
| Assay Time: | 50 min |
| Assay Type: | Quantitative |
| Synonyms: | HYP |
| Detection Instrument: | Microplate reader (550-570 nm, optimum wavelength: 558 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 0.04 μg/mL |
| Detection Range: | 0.04-10 μg/mL |
| Inter-assay CV: | 0.044 |
| Intra-assay CV: | 0.012 |
The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.
| Storage: | 2-8°C |
| Valid Period: | 12 months |
| Other Reagents Required: | 6 mol/L Hydrochloric acid, Concentrated hydrochloric acid (12 mol/L), N-propyl alcohol |
| Other Instruments Required: | Test tube, Glass tube, pH test strips. |
