The IARS Antibody (CAB10190) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blotting applications. By binding to the IARS protein, this antibody allows for the detection and analysis of IARS in various cell types, making it ideal for studies in molecular biology and cell biology.IARS, also known as isoleucyl-tRNA synthetase, plays a crucial role in the translation of genetic information into proteins.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
IARS Antibody
SKU:
CAB10190
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IRS, IARS, ILRS, ILERS, GRIDHH, PRO0785
Positive Sample:
HeLa, HepG2, HT-29, HL-60, Jurkat
Cellular Localization:
Cytoplasm.
Calculated MW:
144kDa
Observed MW:
144kDa
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found.
Purification Method
Affinity purification
Gene ID
3376
RRID
AB_2757712
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using IARS Rabbit pAb (CAB10190) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
