The ID4 Antibody (CAB16229) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting ID4 in human samples, making it ideal for use in Western blot applications. By targeting the ID4 protein, this antibody allows for precise detection and analysis in a variety of cell types, providing insights into its role in processes such as stem cell maintenance, tumorigenesis, and differentiation pathways.ID4, a member of the inhibitor of DNA binding (ID) family, is known for its involvement in multiple cellular processes, including cell proliferation, differentiation, and survival. Dysregulation of ID4 expression has been linked to various diseases, including cancer, neurodevelopmental disorders, and reproductive system abnormalities.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
ID4 Antibody
SKU:
CAB16229
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IDB4, bHLHb27, ID4
Positive Sample:
Mouse kidney
Cellular Localization:
Cytoplasm, Nucleoplasm, Nucleus.
Calculated MW:
17kDa
Observed MW:
20kDa
This gene encodes a member of the inhibitor of DNA binding (ID) protein family. The encoded protein lacks DNA binding ability, and instead regulates gene expression through binding to and inhibiting basic helix-loop-helix transcription factors. This protein has been implicated in the regulation of diverse cellular processes that play a role in development and tumorigenesis.
Purification Method
Affinity purification
Gene ID
3400
RRID
AB_2769909
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse kidney, using ID4 Rabbit pAb (CAB16229) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
