The IFNGR1 Antibody (CAB5748) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting IFNGR1 in human samples, making it an ideal choice for Western blot applications.IFNGR1 is a crucial component of the interferon gamma signaling pathway, which is essential for the immune system's ability to fight off pathogens and tumor cells. Dysregulation of this pathway has been linked to various diseases, including autoimmune disorders and certain types of cancer.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IFNGR1 Antibody
SKU:
CAB5748
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD119, IFNGR, IMD27A, IMD27B, IFNGR1
Positive Sample:
293T, Mouse liver, Rat liver
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
54kDa
Observed MW:
80-90kDa
This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.
Purification Method
Affinity purification
Gene ID
3459
RRID
AB_2766502
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using IFNGR1 Rabbit pAb (CAB5748) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Rat spleen using IFNGR1 Rabbit pAb (CAB5748) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
