The IKKgamma Antibody (CAB0917) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-raised antibody is highly specific for human samples and has been validated for use in Western blot applications, enabling detection and analysis of IKBKG protein levels in a variety of cell types.IKBKG, also known as NEMO (NF-κB Essential Modulator), is crucial for the activation of NF-κB transcription factors, which play a central role in immune responses, inflammation, and cell survival. Dysregulation of the NF-κB pathway is associated with various diseases, including cancer, inflammatory disorders, and autoimmune conditions.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IKKgamma Antibody
SKU:
CAB0917
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
VGSE RKRG MQLE DLKQ QLQQ AEEA LVAK QEVI DKLK EEAE QHKI VMET VPVL KAQA DIYK ADFQ AERQ AREK LAEK KELL QEQL EQLQ REYS KLKA SCQE SARI EDMR KRHV EVSQ APLP PAPA YLSS PLAL PSQR RSPP E
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:1000 - 1:5000
IHC-P
1:50 - 1:100
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.
Purification Method
Affinity purification
Gene ID
8517
RRID
AB_2757460
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using IKKγ Rabbit pAb (CAB0917) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Western blot analysis of lysates from Rat brain, using IKKγ Rabbit pAb (CAB0917) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of U-2 OS cells using IKKγ Rabbit pAb (CAB0917) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
