The IKKbeta Monoclonal Antibody (CAB22425) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in a mouse model, shows high specificity and sensitivity in detecting IKK in human samples, making it suitable for Western blot applications.IKK, also known as IκB kinase, plays a crucial role in the activation of NF-κB, a transcription factor involved in the expression of genes relevant to immune and inflammatory responses.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
IKKbeta Monoclonal Antibody
SKU:
CAB22425
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0085
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene.
Purification Method
Affinity purification
Gene ID
3551
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using IKKβ Rabbit mAb (CAB22425) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using IKKβ Rabbit mAb (CAB22425) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
