The IKZF2 Antibody (CAB12265) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blotting applications. By targeting the IKZF2 protein, this antibody allows for the detection and analysis of IKZF2 expression in a variety of cell types, making it an essential reagent for studies in immunology and cancer research.IKZF2, also known as Helios, plays a crucial role in the regulation of immune responses, particularly in the differentiation and function of T cells.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IKZF2 Antibody
SKU:
CAB12265
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ANF1A2, HELIOS, ZNF1A2, ZNFN1A2, IKZF2
Positive Sample:
K-562, HeLa, Mouse thymus, Rat thymus
Cellular Localization:
Nucleus.
Calculated MW:
60kDa
Observed MW:
60kDa
This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.
Purification Method
Affinity purification
Gene ID
22807
RRID
AB_2759132
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using IKZF2 Rabbit pAb (CAB12265) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
