The IL1beta Monoclonal Antibody (CAB22257) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. RRID Gene ID 16176 Swiss Prot Synonym Il-1b; IL-1beta; IL1β
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
IL1beta Monoclonal Antibody
SKU:
CAB22257
Size:
100μL, 20μL
Reactivity:
Mouse
Clone Number:
ARC56180
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:200 - 1:800
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Il-1b, IL-1beta, IL1β
Positive Sample:
Recombinant Mouse IL-1 beta Protein, Raw 264.7 treated with LPS
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. RRID Gene ID 16176 Swiss Prot Synonym Il-1b; IL-1beta; IL1β
Purification Method:
Affinity purification
Gene ID:
16176
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of Recombinant Mouse IL-1 beta Protein (RP01340), using IL1β Rabbit mAb (CAB22257) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20 s.
Western blot analysis of lysates from RAW 264.7 cells using IL1β Rabbit mAb (CAB22257) at 1:1000 dilution incubated overnight at 4℃. Raw264.7 cells were treated with LPS (1 μg/ml) at 37℃ for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
