Description
INCENP Antibody (CAB0622)
The INCENP Antibody (CAB0622) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, is highly specific for human samples and has been extensively validated for use in Western blot applications.INCENP is essential for proper chromosome segregation and cytokinesis during cell division, making it a key target for studies in cell biology and cancer research. The INCENP Polyclonal Antibody binds specifically to the INCENP protein, allowing for precise detection and analysis in a variety of cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
| Product Name: | INCENP Antibody |
| SKU: | CAB0622 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | ALNV TVDV QSPA CTSY QMTP QGHR APPK INPD NYGM DLNS DDST DDEA HPRK PIPT WARG TPLS QAII HQYY HPPN LLEL FGTI LPLD LEDI FKKS KPRY HKRT SSAV WNSP PLQG ARVP SSLA YSLK KH | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | INCENP |
| Positive Sample: | HeLa, Jurkat, Rat thymus |
| Cellular Localization: | Chromosome, Cytoplasm, Nucleus, Centromere, Cytoskeleton, Kinetochore, Spindle. |
| Calculated MW: | 105kDa |
| Observed MW: | 120-140kDa |
In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).
| Purification Method | Affinity purification |
| Gene ID | 3619 |
| RRID | AB_2757298 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using INCENP Rabbit pAb (CAB0622) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s. |
