The ING4 Antibody (CAB5833) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and is validated for use in Western blot applications. It binds to the ING4 protein, allowing for accurate detection and analysis in various cell types, making it an ideal choice for studies in cancer research and molecular biology.ING4 is a key player in regulating cell growth, apoptosis, and DNA repair, making it a crucial target for investigating cancer development and progression. Its tumor-suppressive properties suggest its potential as a therapeutic target for cancer treatment.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ING4 Antibody
SKU:
CAB5833
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
my036, p29ING4, ING4
Positive Sample:
HeLa, 293T, Raji, A-549, Mouse spleen
Cellular Localization:
Nucleus.
Calculated MW:
29kDa
Observed MW:
29kDa
This gene encodes a tumor suppressor protein that contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in the TP53-dependent regulatory pathway. Multiple alternatively spliced transcript variants have been observed that encode distinct proteins.
Purification Method
Affinity purification
Gene ID
51147
RRID
AB_2766583
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using ING4 Rabbit pAb (CAB5833) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
