The Insig1 Polyclonal Antibody (CAB16278) is a valuable tool for researchers studying Insig1, a key regulator of cholesterol homeostasis and lipid metabolism. This antibody, produced in rabbits, exhibits high specificity and sensitivity for Insig1 in human samples, making it ideal for Western blot applications.Insig1 is a critical player in the cellular response to cholesterol levels, functioning as a sensor that modulates the synthesis and uptake of cholesterol. Dysregulation of Insig1 has been linked to various metabolic disorders, including hypercholesterolemia and non-alcoholic fatty liver disease.
By targeting Insig1, researchers can gain insights into the mechanisms underlying cholesterol regulation and develop novel strategies for treating metabolic diseases. The Insig1 Polyclonal Antibody offers a reliable and accurate tool for investigating Insig1 function in diverse experimental settings, making it a valuable asset for studies in lipid metabolism and related fields.
Product Name:
INSIG1 Rabbit Polyclonal Antibody
SKU:
CAB16278
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human INSIG1 (NP_005533.2).
This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3638
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from rat brain, using INSIG1 Rabbit pAb (CAB16278) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 150s.
