The Insulin Receptor Antibody (CAB16900) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blotting applications. By binding to the insulin receptor protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for studies in diabetes, obesity, and other metabolic disorders.The insulin receptor plays a crucial role in regulating glucose and lipid metabolism, making it a target of interest in understanding diseases like type 2 diabetes and metabolic syndrome.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Insulin Receptor Antibody
SKU:
CAB16900
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HHF5, CD220, Insulin Receptor
Positive Sample:
HepG2, HeLa, Mouse brain, Mouse kidney
Cellular Localization:
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
156kDa
Observed MW:
110kDa/220kDa
This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
3643
RRID
AB_2769983
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Insulin Receptor Rabbit pAb (CAB16900) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg Insulin Receptor antibody (CAB16900). Western blot was performed from the immunoprecipitate using Insulin Receptor (CAB16900) at a dilution of 1:1000.
