The IL3 Antibody (CAB5671) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. It binds specifically to the IL-3 protein, allowing for accurate detection and analysis in various cell types, making it ideal for studies in immunology, hematology, and inflammation research.IL-3 is a key cytokine involved in the growth and differentiation of multiple hematopoietic cell lineages, making it a crucial target for research in various diseases such as leukemia, autoimmune disorders, and inflammatory conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
IL3 Antibody
SKU:
CAB5671
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IL-3, MCGF, MULTI-CSF, IL3
Positive Sample:
Raji, Mouse thymus
Cellular Localization:
Secreted.
Calculated MW:
17kDa
Observed MW:
17kDa
The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
Purification Method
Affinity purification
Gene ID
3562
RRID
AB_2766431
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using IL3 Rabbit pAb (CAB5671) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
