The INTS10 Antibody (CAB6632) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blot applications. INTS10, a subunit of the integrator complex, plays a key role in the proper functioning of RNA polymerase II transcription and RNA processing. Dysregulation of INTS10 has been implicated in diseases such as cancer and neurodevelopmental disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
INTS10 Antibody
SKU:
CAB6632
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
INT10, C8orf35, INTS10
Positive Sample:
A-549, HL-60, HepG2, THP-1, Mouse kidney, Rat testis
Cellular Localization:
Nucleus.
Calculated MW:
82kDa
Observed MW:
70-75kDa
INTS10 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).
Purification Method
Affinity purification
Gene ID
55174
RRID
AB_2767221
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using INTS10 Rabbit pAb (CAB6632) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
