Invertase Activity Assay Kit
Invertase (b-fructofuranosidase, EC 126.96.36.199) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertases cleave at the O-C(fructose) bond, whereas a related enzyme sucrase (EC 188.8.131.52) cleaves at the O-C(glucose) bond. A wide range of microorganisms produce invertase and can, thus, utilize sucrose as a nutrient. Invertase assay finds wide applications in environmental (e.g. soil), agricultural and food (confectionery) industry.Assay Genie's Invertase activity Kit provides a convenient and ultra-sensitive colorimetric and fluorimetric means to measure invertase activity. In the assay, invertase cleaves sucrose, resulting in the formation of fructose and glucose, which is determined by a colorimetric (570nm) or fluorimetric method (Ex/Em = 530/585nm). The assay is simple, sensitive, stable and high-throughput adaptable.
ApplicationsFor quantitative determination of invertase/sucrase activity
- Safe. Non-radioactive assay.
- Sensitive and accurate. As low as 0.007 U/L invertase activity can be quantified.
- Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
- Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Invertase Activity Assay Kit Information:
|Kit Includes: ||10x Reaction Buffer: 12 mL (pH 4.5), 10x Sucrose: 1.5 mL, Assay Buffer: 10 mL, Enzyme Mix: 120 mL Glucose Standard: 1 mL Dye Reagent: 120 mL|
|Kit Requires:||Pipetting devices, centrifuge tubes, clear or black flat bottom 96-well plate.|
|Method of Detection:||OD570nm, or FL530/585nm|
|Detection Limit:||0.007 U/L|
|Samples:||Biological, environment (soil) etc|
|Protocol Length:||40 min|
|Storage:||Store Reaction Buffer and Assay Buffer at 4°C and other reagents at - 20°C.|
|Shelf Life:||6 months|