The IPPK Antibody (PAC023768) is a polyclonal antibody designed for research involving inositol pentakisphosphate 2-kinase (IP5K), an enzyme involved in regulating inositol phosphate metabolism. The antibody, raised in rabbits, is highly specific and reactive with human samples, and has been validated for use in various applications including Western blot and immunohistochemistry.IP5K plays a crucial role in the synthesis of inositol pyrophosphates, which are important signaling molecules involved in diverse cellular processes such as cell growth, apoptosis, and vesicle trafficking.
Dysregulation of inositol phosphate metabolism has been implicated in various diseases including cancer, diabetes, and neurodegenerative disorders.The IPPK Antibody enables the detection and analysis of IP5K in different cell types, making it an invaluable tool for researchers studying the role of inositol phosphate signaling in health and disease. Understanding the function of IP5K and its impact on cellular processes may provide important insights for developing targeted therapies for diseases associated with dysregulated inositol phosphate metabolism.
Synthesized peptide derived from internal of human IPPK.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from RAW cells,K562 cells, LOVO cells and 3T3 cells, using IPPK antiobdy.
Immunofluorescence analysis of HepG2 cells, using IPPK antibody.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using IPPK antibody.
Background:
Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-alpha-induced apoptosis.
Synonyms:
EC 2.7.1.158; Inositol-1; 3; 4; 5
UniProt Protein Function:
IPPK: Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-alpha-induced apoptosis. Belongs to the IPK1 type 2 family.Protein type: Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.158; Kinase, otherChromosomal Location of Human Ortholog: 9q22.31Cellular Component: nucleoplasm; intracellular; cytosolMolecular Function: inositol pentakisphosphate 2-kinase activity; ATP bindingBiological Process: inositol phosphate metabolic process
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a kinase that phosphorylates position 2 of inositol-1,3,4,5,6-pentakisphosphate to form inositol-1,2,3,4,5,6-hexakisphosphate (InsP6). InsP6 has a variety of functions, including stimulation of DNA repair, endocytosis, and mRNA export. [provided by RefSeq, Nov 2010]
