Description
Irf8 Antibody (PACO27665)
The IRF8 Antibody (PACO27665) is a valuable tool for researchers studying the IRF8 protein, a transcription factor known for its role in regulating immune responses and inflammation. This polyclonal antibody, developed using rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to the IRF8 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential tool for studies in immunology and cancer research.
IRF8 is a key player in immune system regulation, controlling the development and function of immune cells. Dysregulation of IRF8 has been linked to various diseases, including cancer and autoimmune disorders. By understanding the function of IRF8, researchers can uncover new insights into disease mechanisms and potentially develop targeted therapies. The IRF8 Antibody is a crucial resource for investigating the role of IRF8 in these contexts and advancing our understanding of immune system function.
| Antibody Name: | Irf8 Antibody (PACO27665) |
| Antibody SKU: | PACO27665 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000 |
| Species Reactivity: | Mouse, Human |
| Immunogen: | Recombinant Mouse Interferon regulatory factor 8 protein (1-424AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot All lanes: Irf8 antibody at 2µg/ml + Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49 kDa Observed band size: 49 kDa . |
| Background: | Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a regulatory role in cells of the immune system. Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes. |
| Synonyms: | Interferon regulatory factor 8 (IRF-8) (Interferon consensus sequence-binding protein) (ICSBP), Irf8, Icsbp Icsbp1 |
| UniProt Protein Function: | IRF8: Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with COPS2. By IFNG/IFN-gamma. Predominantly expressed in lymphoid tissues. Belongs to the IRF family.Protein type: Transcription factor; DNA-bindingCellular Component: nucleoplasm; nucleusMolecular Function: protein binding; DNA binding; transcription factor activityBiological Process: positive regulation of interferon-gamma production; response to bacterium; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of interleukin-12 production; positive regulation of transcription, DNA-dependent; defense response to bacterium; immune response; myeloid cell differentiation; defense response to protozoan; phagocytosis |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene is a transcription factor that belongs to the interferon regulatory factor family. Proteins belonging to this family have a DNA binding domain at the amino terminus that contains five well-conserved tryptophan-rich repeats. This domain recognizes DNA sequences similar to the interferon-stimulated response element. The protein encoded by this gene promotes or suppresses lineage-specific genes to regulate the differentation of lymphoid and myeloid lineage cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] |
| UniProt Code: | P23611 |
| NCBI GenInfo Identifier: | 6680339 |
| NCBI Gene ID: | 15900 |
| NCBI Accession: | NP_032346.1 |
| UniProt Secondary Accession: | P23611 |
| UniProt Related Accession: | P23611 |
| Molecular Weight: | 48,237 Da |
| NCBI Full Name: | interferon regulatory factor 8 |
| NCBI Synonym Full Names: | interferon regulatory factor 8 |
| NCBI Official Symbol: | Irf8Â Â |
| NCBI Official Synonym Symbols: | Myls; ICSBP; IRF-8; Icsbp1; AI893568Â Â |
| NCBI Protein Information: | interferon regulatory factor 8; interferon consensus sequence-binding protein; interferon consensus sequence binding protein 1 |
| UniProt Protein Name: | Interferon regulatory factor 8 |
| UniProt Synonym Protein Names: | Interferon consensus sequence-binding protein; ICSBP |
| Protein Family: | Interferon regulatory factor |
| UniProt Gene Name: | Irf8Â Â |
| UniProt Entry Name: | IRF8_MOUSE |
