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Anti-IRF8 Antibody (CAB5798)

SKU:
CAB5798
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy
$167 - $503
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Description

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Antibody Name:Anti-IRF8 Antibody
Antibody SKU:CAB5798
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 137-426 of human IRF8 (NP_002154.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HL-60, THP-1, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 137-426 of human IRF8 (NP_002154.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ECGR SEID ELIK EPSV DDYM GMIK RSPS PPEA CRSQ LLPD WWAQ QPST GVPL VTGY TTYD AHHS AFSQ MVIS FYYG GKLV GQAT TTCP EGCR LSLS QPGL PGTK LYGP EGLE LVRF PPAD AIPS ERQR QVTR KLFG HLER GVLL HSSR QGVF VKRL CQGR VFCS GNAV VCKG RPNK LERD EVVQ VFDT SQFF RELQ QFYN SQGR LPDG RVVL CFGE EFPD MAPL RSKL ILVQ IEQL YVRQ LAEE AGKS CGAG SVMQ APEE PPPD QVFR MFPD ICAS HQRS FFRE NQQI TV
Gene ID:3394
Uniprot:Q02556
Cellular Location:Nucleus
Calculated MW:48kDa
Observed MW:53kDa
Synonyms:IRF8, H-ICSBP, ICSBP, ICSBP1, IMD32A, IMD32B, IRF-8
Background:Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection.
UniProt Protein Function:IRF8: Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with COPS2. By IFNG/IFN-gamma. Predominantly expressed in lymphoid tissues. Belongs to the IRF family.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: nucleoplasm; cytosol

Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of interferon-gamma production; positive regulation of interleukin-12 production; cytokine and chemokine mediated signaling pathway; defense response to bacterium; myeloid cell differentiation; immune response; negative regulation of transcription from RNA polymerase II promoter; defense response to protozoan; phagocytosis

Disease: Monocyte And Dendritic Cell Deficiency, Autosomal Recessive; Immunodeficiency 32a

NCBI Summary:Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. [provided by RefSeq, Jul 2008]
UniProt Code:Q02556
NCBI GenInfo Identifier:6016308
NCBI Gene ID:3394
NCBI Accession:Q02556.2
UniProt Secondary Accession:Q02556,A0AV82,
UniProt Related Accession:Q02556
Molecular Weight:Calculated MW: 48kDaObserved MW: 53kDa
NCBI Full Name:Interferon regulatory factor 8
NCBI Synonym Full Names:interferon regulatory factor 8
NCBI Official Symbol:IRF8  
NCBI Official Synonym Symbols:ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP  
NCBI Protein Information:interferon regulatory factor 8; interferon consensus sequence binding protein 1
UniProt Protein Name:Interferon regulatory factor 8
UniProt Synonym Protein Names:Interferon consensus sequence-binding protein; H-ICSBP; ICSBP
Protein Family:Interferon regulatory factor
UniProt Gene Name:IRF8  
UniProt Entry Name:IRF8_HUMAN
Anti-IRF8 Antibody (CAB5798)Western blot analysis of extracts of various cell lines, using IRF8 antibody (CAB5798) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 15s.

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