The ISG20 Antibody (CAB14744) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ISG20 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in virology and immunology.ISG20, also known as interferon-stimulated gene 20, plays a crucial role in the antiviral immune response by inhibiting viral replication and promoting cell death in infected cells.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ISG20 Antibody
SKU:
CAB14744
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD25, HEM45, ISG20
Positive Sample:
Raji, A-549, MCF7, Mouse spleen, Rat spleen
Cellular Localization:
Cajal Body, Cytoplasm, Nucleus, Nucleolus.
Calculated MW:
20kDa
Observed MW:
20kDa
Enables 3'-5' exonuclease activity and RNA binding activity. Involved in defense response to virus; negative regulation of viral genome replication; and nucleobase-containing compound catabolic process. Located in cytoplasm and nuclear lumen.
Purification Method
Affinity purification
Gene ID
3669
RRID
AB_2761621
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ISG20 Rabbit pAb (CAB14744) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
