The IST1 Antibody (CAB9401) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit immunization, exhibits high specificity for IST1 in human samples and has been validated for use in Western blot applications. By binding to the IST1 protein, this antibody enables accurate detection and analysis of IST1 in various cell types, making it ideal for investigations in cell biology and molecular biology.IST1 is a key player in cell division and vesicle trafficking, making it a crucial target for research on cellular processes and organelle dynamics.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IST1 Antibody
SKU:
CAB9401
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
9798
RRID
AB_2770001
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using IST1 Rabbit pAb (CAB9401) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.