The Integrin beta5/ITGB5 Monoclonal Antibody (CAB23428) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in mice, is highly specific for human samples and has been validated for use in Western blot and immunohistochemistry applications.Integrin α5/ITGB5 is a member of the integrin family of proteins, which play important roles in cell adhesion and signaling. It is involved in processes such as angiogenesis, wound healing, and tumor metastasis, making it a target of interest in cancer research and vascular biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Integrin beta5/ITGB5 Monoclonal Antibody
SKU:
CAB23428
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC60869
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:4000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ITGB5, integrin beta-5, Integrin β5/ITGB5
Positive Sample:
HT-1080, A549
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
88kDa
Observed MW:
100kDa
This gene encodes a beta subunit of integrin, which can combine with different alpha chains to form a variety of integrin heterodimers. Integrins are integral cell-surface receptors that participate in cell adhesion as well as cell-surface mediated signaling. The alphav beta5 integrin is involved in adhesion to vitronectin.
Purification Method
Affinity purification
Gene ID
3693
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Integrin β5/ITGB5 Rabbit mAb (CAB23428) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
