The ITK/EMT Monoclonal Antibody (CAB22617) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced from mouse hybridoma cells, exhibits high specificity and sensitivity in detecting ITK in human samples, making it ideal for Western blot, immunohistochemistry, and flow cytometry applications.ITK, also known as interleukin-2-inducible T-cell kinase, is essential for T cell receptor signaling and immune response regulation. Its role in modulating T cell function makes it a key player in diseases such as cancer, autoimmune disorders, and infectious diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ITK/EMT Monoclonal Antibody
SKU:
CAB22617
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC58362
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:6000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EMT, LYK, LPFS1, PSCTK2, ITK/EMT
Positive Sample:
Jurkat, Molt-4
Cellular Localization:
Cytoplasm.
Calculated MW:
72kDa
Observed MW:
75kDa
This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.
Purification Method
Affinity purification
Gene ID
3702
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ITK/EMT Rabbit mAb (CAB22617) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
