Description
JMY Antibody (PACO56078)
The JMY Antibody (PAC056078) is a high-quality polyclonal antibody designed for research involving JMY, a protein known for its roles in cell migration, adhesion, and cytoskeleton regulation. This antibody, produced using rabbit immunization, is highly specific to human samples and is validated for use in Western blot and immunofluorescence applications.JMY, also known as Junction Mediating and Regulatory Protein, is a key regulator of actin dynamics and cell motility, making it a crucial player in processes such as wound healing, embryonic development, and cancer metastasis.
Its functions in cell migration and adhesion make it a significant target for research aimed at understanding the mechanisms underlying these processes.The JMY Antibody enables precise detection and analysis of JMY protein levels in various cell types, making it an invaluable tool for studies in cell biology, developmental biology, and cancer research. By investigating the function of JMY, researchers can gain insights into the molecular mechanisms governing cell movement and potentially identify new therapeutic targets for diseases characterized by abnormal cell motility.
| Antibody Name: | JMY Antibody (PACO56078) |
| Antibody SKU: | PACO56078 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Junction-mediating and -regulatory protein (708-848AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO56078 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO56078 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity). |
| Synonyms: | Junction-mediating and -regulatory protein, JMY |
| UniProt Protein Function: | JMY: Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location. Belongs to the JMY family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Apoptosis; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 5q14.1 Cellular Component: leading edge; nucleoplasm; nucleus Molecular Function:protein binding; transcription coactivator activity Biological Process: cell cycle arrest; positive regulation of apoptosis; positive regulation of transcription factor activity; regulation of transcription from RNA polymerase II promoter |
| UniProt Code: | Q8N9B5 |
| NCBI GenInfo Identifier: | 172045777 |
| NCBI Gene ID: | 133746 |
| NCBI Accession: | Q8N9B5.2 |
| UniProt Secondary Accession: | Q8N9B5,A1L4P5, B5MDS2, B5MDT0, |
| UniProt Related Accession: | Q8N9B5 |
| Molecular Weight: | 110,280 Da |
| NCBI Full Name: | Junction-mediating and -regulatory protein |
| NCBI Synonym Full Names: | junction mediating and regulatory protein, p53 cofactor |
| NCBI Official Symbol: | JMYÂ Â |
| NCBI Official Synonym Symbols: | WHAMM2; WHDC1L3Â Â |
| NCBI Protein Information: | junction-mediating and -regulatory protein |
| UniProt Protein Name: | Junction-mediating and -regulatory protein |
| Protein Family: | Junction-mediating and -regulatory protein |
| UniProt Gene Name: | JMYÂ Â |
| UniProt Entry Name: | JMY_HUMAN |
| Antibodies |
| JMY Antibody (PACO00989) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
