The JNK1/JNK3 Monoclonal Antibody (CAB5051) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through hybridoma technology, is highly specific for these two isoforms, allowing for accurate detection and analysis in various cell types and tissues.JNK1 and JNK3 are members of the c-Jun N-terminal kinase family, known for their roles in stress response, apoptosis, and inflammation. Dysregulation of JNK signaling has been implicated in various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
JNK1/JNK3 Monoclonal Antibody
SKU:
CAB5051
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1163
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]
Purification Method
Affinity purification
Gene ID
5599 5602
RRID
AB_2863424
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using JNK1/JNK3 Rabbit mAb (CAB5051) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1min.
Immunohistochemistry analysis of paraffin-embedded Human colon using JNK1/JNK3 Rabbit mAb (CAB5051) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using JNK1/JNK3 Rabbit mAb (CAB5051) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using JNK1/JNK3 Rabbit mAb (CAB5051) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
