The KIF20B Antibody (CAB15360) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables several functions, including WW domain binding activity; plus-end-directed microtubule motor activity; and protein homodimerization activity. Involved in positive regulation of cell population proliferation and positive regulation of cytokinesis. Located in several cellular components, including microtubule cytoskeleton; midbody; and nuclear lumen.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KIF20B Antibody
SKU:
CAB15360
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:200 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CT90, MPP1, KRMP1, MPP-1, MPHOSPH1, KIF20B
Positive Sample:
U-251MG, OVCAR3, 293T, HT-29, NIH/3T3, Mouse brain, Rat brain
Cellular Localization:
Cell Projection, Cytoplasm, Midbody, Nucleus, Axon, Centrosome, Cytoskeleton, Growth Cone, Microtubule Organizing Center, Nucleolus, Nucleoplasm, Spindle, Spindle Pole.
Calculated MW:
211kDa
Observed MW:
210kDa
Enables several functions, including WW domain binding activity; plus-end-directed microtubule motor activity; and protein homodimerization activity. Involved in positive regulation of cell population proliferation and positive regulation of cytokinesis. Located in several cellular components, including microtubule cytoskeleton; midbody; and nuclear lumen.
Purification Method
Affinity purification
Gene ID
9585
RRID
AB_2762262
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using KIF20B Rabbit pAb (CAB15360) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
