The KIF23 Monoclonal Antibody (CAB4896) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in multiple transcript variants.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
KIF23 Monoclonal Antibody
SKU:
CAB4896
Size:
100μL, 20μL
Reactivity:
Human, Rat
Clone Number:
ARC0335
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
9493
RRID
AB_2863380
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using KIF23 Rabbit mAb (CAB4896) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg KIF23 Rabbit mAb (CAB4896). Western blot was performed from the immunoprecipitate using KIF23 Rabbit mAb (CAB4896) at a dilution of 1:1000.
