The KLF14 Antibody (CAB18607) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By binding specifically to the KLF14 protein, this antibody enables the detection and analysis of KLF14 in various cell types, making it an ideal choice for studies in molecular biology and cancer research.KLF14 is a key player in the control of gene expression, influencing a wide range of biological functions such as cell proliferation, differentiation, and metabolism.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KLF14 Antibody
SKU:
CAB18607
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BTEB5, KLF14
Positive Sample:
HT-29, Mouse stomach, Rat stomach
Cellular Localization:
Nucleus.
Calculated MW:
33kDa
Observed MW:
33kDa
This intronless gene encodes a member of the Kruppel-like family of transcription factors. The encoded protein functions as a transcriptional co-repressor, and is induced by transforming growth factor-beta (TGF-beta) to repress TGF-beta receptor II gene expression. This gene exhibits imprinted expression from the maternal allele in embryonic and extra-embryonic tissues.
Purification Method
Affinity purification
Gene ID
136259
RRID
AB_2862364
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using KLF14 Rabbit pAb (CAB18607) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from HT-29 cells, using KLF14 Rabbit pAb (CAB18607) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
