The KLF4 Polyclonal Antibody (CAB21709) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and is highly specific for human samples, making it ideal for use in various research applications including Western blotting.KLF4, also known as Krüppel-like factor 4, is a key regulator of gene expression and has been implicated in a wide range of biological processes, including stem cell pluripotency, cancer progression, and tissue regeneration. By targeting KLF4 with this antibody, researchers can gain valuable insights into its function and potential therapeutic applications in diseases such as cancer and stem cell disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
KLF4 Polyclonal Antibody
SKU:
CAB21709
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EZF, GKLF, KLF4
Positive Sample:
Hela, HCT 116, HT-29
Cellular Localization:
Nucleus.
Calculated MW:
55kDa
Observed MW:
Refertofigures
This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
9314
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using KLF4 Rabbit pAb (CAB21709) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
