The KLHL8 Antibody (CAB14917) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in various applications, including Western blot and immunofluorescence.KLHL8 is a Kelch-like ECH-associated protein 1 (Keap1) interacting protein that regulates the Keap1-Nrf2 pathway, which is involved in the cellular response to oxidative stress. The antibody binds specifically to the KLHL8 protein, allowing for accurate detection and analysis in different cell types.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KLHL8 Antibody
SKU:
CAB14917
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KLHL8
Positive Sample:
293T, Mouse brain, Mouse kidney, Rat thymus
Cellular Localization:
Nucleoplasm.
Calculated MW:
69kDa
Observed MW:
69kDa
Involved in protein ubiquitination and ubiquitin-dependent protein catabolic process. Located in nucleoplasm. Part of Cul3-RING ubiquitin ligase complex.
Purification Method
Affinity purification
Gene ID
57563
RRID
AB_2761798
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using KLHL8 Rabbit pAb (CAB14917) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of C6 cells using KLHL8 Rabbit pAb (CAB14917) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using KLHL8 Rabbit pAb (CAB14917) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
