Description
Anti-SPR Antibody (CAB19859)[KO Validated]
The KO Validated SPR Rabbit Polyclonal Antibody (CAB19859) is a valuable tool for researchers studying CD300A, a cell surface molecule known for its role in immune regulation. This antibody, specifically designed for Western blot applications, is highly reactive with human samples and has been rigorously validated for its specificity and reliability.CD300A, also known as an immune inhibitory receptor, is involved in modulating immune responses and controlling inflammation. By targeting the CD300A protein, researchers can gain valuable insights into immune function and potential therapeutic targets for diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions.
With its high reactivity and validation for use in various cell types, the KO Validated SPR Rabbit Polyclonal Antibody (CAB19859) is a powerful tool for studies in immunology and cancer research. Its ability to detect and analyze CD300A protein expression makes it an essential resource for scientists looking to further understand immune regulation and its implications in disease pathology.
| Antibody Name: | Anti-SPR Antibody [KO Validated] |
| Antibody SKU: | CAB19859 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human SPR. |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant protein of human SPR. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 6697 |
| Uniprot: | P35270 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 28kDa |
| Observed MW: | 28KDa |
![Anti-SPR Antibody [KO Validated] (CAB19859)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/d/962/A19859_1__39150.jpg) | Western blot analysis of extracts from normal (control) and SPR knockout (KO) HeLa cells, using SPR antibody (CAB19859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
| Synonyms: | SPR, SDR38C1 |
| Background: | This gene encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1. |
| UniProt Protein Function: | SPR: Catalyzes the final one or two reductions in tetra- hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin. Defects in SPR are the cause of dystonia DOPA-responsive due to sepiapterin reductase deficiency (DRDSPRD). In the majority of cases, patients manifest progressive psychomotor retardation, dystonia and spasticity. Cognitive anomalies are also often present. The disease is due to severe dopamine and serotonin deficiencies in the central nervous system caused by a defect in BH4 synthesis. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Belongs to the sepiapterin reductase family. |
| UniProt Protein Details: | Protein type:Cofactor and Vitamin Metabolism - folate biosynthesis; EC 1.1.1.153; Oxidoreductase Chromosomal Location of Human Ortholog: 2p13.2 Cellular Component: cytosol; nucleoplasm Molecular Function:aldo-keto reductase activity; NADP binding; sepiapterin reductase activity Biological Process: nitric oxide biosynthetic process; regulation of nitric-oxide synthase activity; tetrahydrobiopterin biosynthetic process Disease: Dystonia, Dopa-responsive, Due To Sepiapterin Reductase Deficiency |
| NCBI Summary: | This gene encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35270 |
| NCBI GenInfo Identifier: | 464801 |
| NCBI Gene ID: | 6697 |
| NCBI Accession: | P35270.1 |
| UniProt Secondary Accession: | P35270,Q53GI9, Q9UBB1, A8K741, D6W5H2, |
| UniProt Related Accession: | P35270 |
| Molecular Weight: | 28kDa |
| NCBI Full Name: | Sepiapterin reductase |
| NCBI Synonym Full Names: | sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) |
| NCBI Official Symbol: | SPRÂ Â |
| NCBI Official Synonym Symbols: | SDR38C1Â Â |
| NCBI Protein Information: | sepiapterin reductase |
| UniProt Protein Name: | Sepiapterin reductase |
| Protein Family: | Sproutin |
| UniProt Gene Name: | SPRÂ Â |
