The [KO Validated] Src Monoclonal Antibody (CAB19119) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated from rabbit monoclonal cells, boasts high specificity and sensitivity towards SRC, ensuring reliable results in experiments such as Western blotting and immunohistochemistry.SRC is a non-receptor tyrosine kinase that regulates various cellular processes, including cell growth, survival, and differentiation. Dysregulation of SRC has been implicated in numerous diseases, particularly cancer, making it a prime target for therapeutic interventions.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] Src Monoclonal Antibody
SKU:
CAB19119
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0378
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Purification Method
Affinity purification
Gene ID
6714
RRID
AB_2862612
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] Src Rabbit mAb (CAB19119) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from wild type (WT) and Src knockout (KO) HeLa cells, using [KO Validated] Src Rabbit mAb (CAB19119) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1min.
Confocal imaging of A549 cells using [KO Validated] Src Rabbit mAb (CAB19119,at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Confocal imaging of NIH/3T3 cells using [KO Validated] Src Rabbit mAb (CAB19119,at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
